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KMID : 1098420230310040211
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Korean Journal of Medicinal Crop Science
2023 Volume.31 No. 4 p.211 ~ p.221
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Radical Scavenging Active Compound Analysis in Extract of Coffee Silver Skin
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Lee Kyoung-In
Back Ji-Hwan
Weon Jong-Hyuck
Choi Chul-Hee
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Abstract
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Background : Coffee silver skin is a by-product generated during the processing of coffee, which has recently been cultivated mainly in the southern regions of Korea. Radical scavenging active compounds were analyzed as part of a study of active compounds present in coffee silver skin.
Methods and Results : Hot water extracts and ethanol extracts were prepared at varying concentrations (10% - 95%). Simultaneously with liquid chromatography (LC) separation, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was measured and mass spectrometry (MS) of the identified radical scavenging active compound peak was performed. The analysis results confirmed the presence of three compounds, eicosanoyl-5-hydroxytryptamide, docosanoyl-5-hydroxytryptamide, and tetracosanoyl-5-hydroxytryptamide. In the rapid LC-MS/MS analysis using the multiple reaction monitoring mode, each active compound showed a higher content as the ethanol concentration increased. In particular, the content of each compound was found to be significantly decreased in the extract with an ethanol concentration of 60% or less.
Conclusions : The three identified alkanoyl-5-hydroxytryptamide compounds are reported to possess antioxidant activity and various neurological disease-related effects. Therefore, this analysis method can be applied to research or product development using coffee silver skin
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KEYWORD
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Coffee Silver Skin, Liquid Chromatography, Mass Spectrometry, Radical Scavenging Activity
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